Protein Separation Using Affinity-Based Membrane Chromatography
Keywords:
Affinity Membrane Chromatography, Protein Separation, Biopharmaceutical Processing, Ligand Binding, Membrane Performance, Selective Adsorption, Downstream Processing, Hybrid Membranes.Abstract
This study investigates the application of affinity-based membrane chromatography (ABMC)
techniques for the efficient protein separation in biopharmaceutical processes. The goal is to improve
membrane functionality to sustain higher perfusion rates by implementing flow-through designs and hybrid
ligand modifications. This work uses computational simulations alongside experimental validation of the
membranes to test their selective adsorption, binding, and bind-release cycles. The findings showed increased
productivity and purity relative to precedented resin-based chromatographic techniques. This research helps
downstream processing of biologics by offering a membrane separation method that is easier to automate
and more efficient than existing solutions.
