Development of Enzyme-Immobilized Nanostructured Biosensors for Rapid Detection of Pesticide Residues in Agricultural Products Data

Abstract

Due to the high demand for safety in agricultural products, there is a requirement for rapid, sensitive and reliable analytical techniques that can be used to detect pesticide residues in food samples. Traditional pesticide detection systems require the use of costly and complicated equipment, sample preparation steps that take a long period of time and special laboratories; thus, they are not suitable for use in rapid monitoring. In this research project, the application of enzyme-immobilized nanostructure biosensors for rapid detection of pesticide residues in agriculture products will be studied. The uniqueness of this technique will be realized through the combination of enzyme immobilization techniques and nanostructure material technology in order to enhance biosensors sensitivity, catalytic efficiency and performance. Acetylcholinesterase (AChE) serves as the main recognition enzyme due to its sensitivity to organophosphate and carbamate insecticides. The biosensors prepared are characterized using electrochemical techniques, calibration curve method, response time determination, selectivity studies, enzyme stability tests, and pesticide recovery studies. Comparative characterization of the biosensor includes detection limit, sensitivity, linear range of detection, response time, reproducibility, and stability of the working biosensor. This study analyzes the dependence of the pesticide detection capability on the composition of the nanomaterials used and the immobilization of the enzyme. It is believed that the proposed biosensor will show high sensitivity, fast response, and increased stability of the enzyme. Food samples such as fruits and vegetables are analyzed for practical application of the pesticide residues. The expected results reveal the feasibility of the biosensors based on nanostructured enzymes.